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COMSOL Inc
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COMSOL Inc
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ANSYS inc
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Image Search Results
Journal: Small (Weinheim an Der Bergstrasse, Germany)
Article Title: Vascular Microphysiological System for Investigating Endothelial Barrier Function During Organ Preservation and Reperfusion
doi: 10.1002/smll.202410168
Figure Lengend Snippet: 3D vascular MPS with variable wall shear stress (WSS) outfitted for electrical impedance spectroscopy (EIS). A) Schematic of PDMS MPS assembly with the electrode arrays positioned along the X‐axis. B) Photograph of vascular MPS instrumented with 40 µm width electrodes S1 through S4 at 4 regions within the tissue chamber at different wall shear stresses. Outline of cell chamber is marked with fiduciary black lines. Scale bar: 1 mm. C) Finite element analysis using COMSOL modeling showing: i) fluid velocity profiles in the tapered cell chamber; ii) WSS along the X ‐axis at Y = 0; and, iii) Y ‐axis WSS at X = 0.5, 3.5, and 6.5 mm with a media volumetric flowrate ( Q ) of 40 µL min −1 . The dotted lines in ii) correspond to X = 1, 3.5, and 6 mm. D) Confocal immunofluorescence images of human coronary artery ECs within the cell chamber of the vascular MPS. ECs organize according to shear stress: i) at point 1 in Ci, low shear stress cells are disorganized; and, ii) at point 2 in Ci, high shear stress cells are aligned with flow and have larger volume. Images on the right (red arrows) demonstrate ECs cover the surface area of the cell channel, clearly developing a 3D open lumen. VE‐Cadherin (green) and nuclei (DAPI/blue). Scale bar: 100 µm.
Article Snippet: WSS was taken from the shear rate from the
Techniques: Shear, Impedance Spectroscopy, Immunofluorescence